Journal: bioRxiv
Article Title: Immunotherapy of cytomegalovirus infection by low-dose adoptive transfer of antiviral CD8 T cells relies on substantial post-transfer expansion of central memory cells but not effector-memory cells
doi: 10.1101/2023.08.31.555660
Figure Lengend Snippet: (A) Frequencies of viral epitope-specific TCM, iTEM, and cTEM. (Left panel) IE1 peptide-specific cells. (Right panel) m164 peptide-specific cells. Frequencies refer to functional cells responding with secretion of IFNγ in an ELISpot assay to stimulation by P815 cells loaded with the respective antigenic peptide at the saturating concentration of 10 -8 M. (NP) no peptide. (B) Cumulative avidity distributions and deduced Gaussian-like avidity distributions of TCM, iTEM, and cTEM specific for antigenic peptides IE1 and m164, corresponding to (A). Stimulator cells in the ELISpot assay were P815 cells loaded with the respective antigenic peptide in the graded concentrations indicated. (NP) no peptide. (EC 50 and 95% CI) effective concentration and its 95% confidence interval of antigenic peptide that leads to the half-maximal response of the CD8 T-cell population tested. Throughout, bars represent frequencies determined by intercept-free linear regression analysis. Error bars represent the 95% confidence intervals. Data for transferred CD8 T-cell subsets are color-coded as defined in .
Article Snippet: Based on MPN and the corresponding upper and lower 95% confidence limits, half-maximal effective concentration (EC 50 ) values, representing peptide concentrations that result in the half-maximal response of the cell population, were calculated with Quest Graph™ EC 50 Calculator (AAT Bioquest, Inc.; retrieved from https://www.aatbio.com/tools/ec50-calculator ).
Techniques: Functional Assay, Enzyme-linked Immunospot, Concentration Assay
